The improvement and standardization of the simplified process for the production of foot and mouth disease virus from BHK suspension cells

Abstract

Improvements have been made to the methodology for the production of foot and mouth disease (FMD) virus from BHK 21 clone 13 suspension cells by the simplified process. Data derived from some 600 individual 8-1 cultures covering all seven types of FMD virus has been analysed. The production of virus was shown to (1) have no direct relationship to cell passage level and (2) to be inversely related to the cell multiplication factor observed during the cell growth cycle immediately prior to infection. © 1983 The International Association of Biological Standardization.