The use of isolated hepatocytes from rainbow trout (salmo gairdneri) in the metabolism of acetaminophen

Abstract

1. 1. Hepatocytes were isolated from rainbow trout. Cytrochrome P-450 and glutathione (GSH) were determined and found to be considerably lower than values reported for rat hepatocytes. 2. 2. Acetaminophen was incubated with rainbow trout hepatocytes; and glucuronide, glutathione and sulfate conjugates were analyzed. 3. 3. The formation of GSH conjugates were lower than that of glucuronide conjugates and both were several fold lower than those reported for rat hepatocytes. 4. 4. In contrast to rat hepatocytes, sulfate conjugates were not detected. 5. 5. These results demonstrate the ability of isolated fish hepatocytes to metabolize xenobiotics and further illustrates differences in the formation of metabolites between trout and mammals. © 1981.