Isolation and characterization of a cDNA from Trichoderma harzianum P1 encoding a 14-3-3 protein homolog

  • Klemsdal S
  • Hayes C
  • Hjeljord L
 et al. 
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Abstract

A full-length cDNA clone, Th1433, (GenBank accession No. U24158), was isolated and characterized from the filamentous fungus, Trichoderma harzianum. The deduced amino acid (aa) sequence showed an acidic 30-kDa protein homologous to the 14-3-3 proteins, a family of putative kinase regulators originally characterized in mammalian brain tissue. The greatest homology, 71% identical aa, was found to BMH1, the corresponding protein from Saccharomyces cerevisiae and to the ε isoform from sheep brain. Southern analysis of genomic DNA indicated that Th1433 is a member of a small genomic family. At least two genes encoding 14-3-3-like proteins exist in T. harzianum. Northern analysis showed the highest level of expression during the first day after inoculation of the culture with conidial spores.

Author-supplied keywords

  • 14-3-3 proteins
  • Nucleotide sequence
  • Protein kinase regulators

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Authors

  • Sonja S. Klemsdal

  • Christopher K. Hayes

  • Linda Hjeljord

  • Matteo Lorito

  • Gary E. Harman

  • Arne Tronsmo

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