A full-length cDNA clone, Th1433, (GenBank accession No. U24158), was isolated and characterized from the filamentous fungus, Trichoderma harzianum. The deduced amino acid (aa) sequence showed an acidic 30-kDa protein homologous to the 14-3-3 proteins, a family of putative kinase regulators originally characterized in mammalian brain tissue. The greatest homology, 71% identical aa, was found to BMH1, the corresponding protein from Saccharomyces cerevisiae and to the ε isoform from sheep brain. Southern analysis of genomic DNA indicated that Th1433 is a member of a small genomic family. At least two genes encoding 14-3-3-like proteins exist in T. harzianum. Northern analysis showed the highest level of expression during the first day after inoculation of the culture with conidial spores.
Klemsdal, S. S., Hayes, C. K., Hjeljord, L., Lorito, M., Harman, G. E., & Tronsmo, A. (1996). Isolation and characterization of a cDNA from Trichoderma harzianum P1 encoding a 14-3-3 protein homolog. Gene, 171(1), 123–127. https://doi.org/10.1016/0378-1119(96)00031-5