Light microscopic depth measurements of thick sections

  • Harris R
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Abstract

Measurements of the thickness of a thick histological section or the depth of an object within the section, can be made most easily by reading differences in the setting of the fine focus knob of a compound light microscope. Two corrections must be made to such measurements: (1) when using a dry objective, a factor of 1.5 for the index of refraction of the specimen, and (2) a correction for the calibration of the fine focus itself. In addition, several precautions should be taken to minimize errors in the readings. © 1985.

Author-supplied keywords

  • Snell's law
  • fine focus
  • light microscopy
  • morphometry
  • oil immersion
  • refractive index
  • section thickness

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Authors

  • Roger M. Harris

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