Light microscopic depth measurements of thick sections

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Abstract

Measurements of the thickness of a thick histological section or the depth of an object within the section, can be made most easily by reading differences in the setting of the fine focus knob of a compound light microscope. Two corrections must be made to such measurements: (1) when using a dry objective, a factor of 1.5 for the index of refraction of the specimen, and (2) a correction for the calibration of the fine focus itself. In addition, several precautions should be taken to minimize errors in the readings. © 1985.

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Harris, R. M. (1985). Light microscopic depth measurements of thick sections. Journal of Neuroscience Methods, 14(2), 97–100. https://doi.org/10.1016/0165-0270(85)90120-7

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