A procedure was developed for preparing cell fractions rich in chloroplasts, nuclei, and pea enation mosaic virus (PEMV)-induced cytopathological structures (vesicles). Those fractions from infected pea plants which contained nuclei or vesicles also contained actinomycin D-insensitive RNA polymerase activity and PEMV-specific hybridizable RNA. The fraction from infected plants containing predominantly chloroplasts had little of this polymerase activity or RNA, as was the case with all fractions from healthy plants. The significance of the polymerase activity in the nuclei and vesicles is discussed, as well as the potential role of the vesicles in the virus infection cycle. © 1977.
CITATION STYLE
Powell, C. A., De Zoeten, G. A., & Gaard, G. (1977). The localization of pea enation mosaic virus-induced RNA-dependent RNA polymerase in infected peas. Virology, 78(1), 135–143. https://doi.org/10.1016/0042-6822(77)90085-X
Mendeley helps you to discover research relevant for your work.