Mechanism of cytotoxicity of aflatoxin B1: Role of cytochrome P1-450

24Citations
Citations of this article
3Readers
Mendeley users who have this article in their library.
Get full text

Abstract

A mouse hepatoma cell line, Hepa-1, is highly sensitive to the toxic effects of Aflatoxin B1 (AFB1). Half maximal survival (LD50) of cells occurs at 0.068 ug AFB1/ml. Benzo(a)anthracene, which induces aryl hydrocarbon hydroxylase and cytochrome P1-450 in Hepa-1, causes a slight increase in the toxicity of AFB1 (LD50 = 0.034 ug/ml). An aryl hydrocarbon hydroxylase- and cytochrome P1-450-deficient mutant of Hepa-1 is, however, over 100 times more resistant to AFB1 than Hepa-1. Almost no decline in survival is observed at 5 ug AFB1/ml. Cytochrome P1-450 thus effects strongly on the cytotoxicity of AFB1 in these cells. The basal activity in Hepa-1 is enough to elicit an almost full toxic effect. AFB1, although a substrate for cytochrome P1-450, does not act as an inducer of aryl hydrocarbon hydroxylase. © 1987.

Cite

CITATION STYLE

APA

Kärenlampi, S. O. (1987). Mechanism of cytotoxicity of aflatoxin B1: Role of cytochrome P1-450. Biochemical and Biophysical Research Communications, 145(2), 854–860. https://doi.org/10.1016/0006-291X(87)91043-6

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free