Membrane-surfactant interactions The effect of triton X-100 on sarcoplasmic reticulum vesicles

  • Prado A
  • Arrondo J
  • Villena A
 et al. 
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Abstract

The effect of Triton X-100 on purified sarcoplasmic reticulum vesicles has been studied by means of chemical, ultrastructural and enzymic techniques. At low detergent/membrane ratios (about 1 Triton X-100 per 60 phospholipid molecules) the only effect observed is an increase in vesicle permeability. Higher surfactant concentrations, up to a 1:1 detergent/phospholipid ratio, produce a large enhancement of ATPase activity. Membrane solubilization occurs as a critical phenomenon when the surfactant/phospholipid molar ratio reaches a value around 1.5:1, corresponding to 2 μmol Triton X-100/mg protein. At this point, the suspension turbidity drops, virtually all the protein and phospholipid is solubilized and every organized structure disappears. Simultaneously, a dramatic increase in the specific activity of the solubilized ATPase is observed. The sudden solubilization of almost all the bilayer components at a given detergent concentration is attributed to the relative simplicity of this membrane system. Solubilization takes place at the same surfactant/membrane ratio, at least between 0.5 and 4 mg membrane protein/ml. The non-solubilized residue seems to consist mainly of delipidized aggregated forms of ATPase. © 1983.

Author-supplied keywords

  • (Rabbit muscle)
  • Detergent-membrane interaction
  • Membrane solubilization
  • Sarcoplasmic reticulum
  • Triton X-100

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Authors

  • A. Prado

  • J. L.R. Arrondo

  • A. Villena

  • F. M. Goñi

  • J. M. Macarulla

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