Analog chromatography with glomerular basement membrane isolated from normal and nephrotic rabbits was performed to see if there was a molecular sieving effect by the basement membrane. In a normal glomerular basement membrane column,125I-labeled human serum globulin and131I-labeled human serum albumin were eluted at the same position, probably at the void volume of the column. However,125I-labeled insulin and tritiated water appeared with a delay and gave separated peaks. In nephrotic glomerular basement membrane,125I-labeled human globulin,131I-labeled serum albumin,125I-labeled insulin and tritiated water gave four distinctly separated peaks in that order. By analogy with Sephadex, the sieving effect of glomerular basement membrane was demonstrated. © 1976.
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