An Na-23 and P-31 NMR investigation of sonicated cardiolipin sodium salt in aqueous medium

  • Lai A
  • Casu M
  • Meloni C
 et al. 
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 2

    Citations

    Citations of this article.

Abstract

Na-23 and P-31 nuclear magnetic resonance was used to investigate the structure and dynamics of Na+trapped in the enclosed aqueous spaces of unilamellar vesicles of Cardiolipin sodium salt. After sonicated Cardiolipin sodium salt forms a clear solution in aqueous medium and gives rise to a narrow, isotropic P-31 NMR line. This line was attributed to the presence of either vesicles or more complex liquid crystalline structures showing superposed signals for inner and outer phosphate groups. The use of paramagnetic shift reagent Dy(PPPi)2made it possible to observe only the line of the phosphate groups arranged within the structures. From an analysis of the spin-lattice relaxation parameters of Na-23 at 21.04 MHz and 52.6 MHz, the correlation time τ = 1.3×10-9sec was estimated. It was then possible to calculate the value of 1.9 MHz for the quadrupolar coupling parameter. These findings were interpreted in terms of the occurrence of specific bindings between Na+and the phosphate moieties within the unilamellar structures of Cardiolipin. © 1989.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A. Lai

  • M. Casu

  • C. Meloni

  • U. Muscatello

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free