3Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Downhill sodium efflux from right-side-out E. coli membrane vesicles was found to be stimulated by negative electric potential, as has been reported earlier [Bassilana et al., Biochemistry 23 (1984) 1015-1022], and in agreement with the concept of electrogenic Na+/nH+antiporters with n > 1. However, sodium efflux was much more accelerated by positive electric potential, indicating the operation of another sodium transport system. ΔpH (alkaline inside), created by a pH shift from 8.5 to 6.8 in the medium was found to drive sodium efflux against its concentration gradient, but only when the vesicles had been loaded with both Na+and K+. Efflux of K+against the concentration gradient was also observed under these conditions. When the vesicles were loaded separately with sodium tricine or potassium tricine, no K+efflux and insignificant Na+efflux were observed. We propose that there are at least two different mechanisms responsible for Na+efflux in E. coli vesicles. One is the Na+/nH+antiporter previously described, and the other is a novel Na+,K+/mH+antiporter. © 1995.

Author supplied keywords

Cite

CITATION STYLE

APA

Verkhovskaya, M. L., Verkhovsky, M. I., & Wikström, M. (1995). A novel antiporter activity catalyzing sodium and potassium transport from right-side-out vesicles of E. coli. FEBS Letters, 363(1–2), 46–48. https://doi.org/10.1016/0014-5793(95)00281-D

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free