Peptide: N-glycosidase F: studies on the glycoprotein aminoglycan amidase from Flavobacterium meningosepticum

  • Mussar K
  • Murray G
  • Martin B
 et al. 
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Abstract

Peptide: N-glycosidase from Flavobacterium meningosepticum was isolated in a homogeneous state and its physico-chemical characterization was accomplished. The reliability of the previously recorded assay procedures was assessed. Using an octaglycopeptide derived from ovomucoid a rapid and sensitive FPLC method was developed for the assay of enzymatic activity. Peptide: N-glycosidase was found to effect deglycosylation of glycoproteins bearing complex and/or multiantennary glycans even in their native state. In contrast, glycoproteins with high mannose and/or hybrid carbohydrates required denaturation to become susceptible to deglycosylation by the enzyme. © 1989.

Author-supplied keywords

  • Glycoproteins
  • N-glycosidase
  • Peptide

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Authors

  • Keith J. Mussar

  • Gary J. Murray

  • Brian M. Martin

  • Thammaiah Viswanatha

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