Peptide: N-glycosidase F: studies on the glycoprotein aminoglycan amidase from Flavobacterium meningosepticum

  • Mussar K
  • Murray G
  • Martin B
 et al. 
  • 5


    Mendeley users who have this article in their library.
  • 14


    Citations of this article.


Peptide: N-glycosidase from Flavobacterium meningosepticum was isolated in a homogeneous state and its physico-chemical characterization was accomplished. The reliability of the previously recorded assay procedures was assessed. Using an octaglycopeptide derived from ovomucoid a rapid and sensitive FPLC method was developed for the assay of enzymatic activity. Peptide: N-glycosidase was found to effect deglycosylation of glycoproteins bearing complex and/or multiantennary glycans even in their native state. In contrast, glycoproteins with high mannose and/or hybrid carbohydrates required denaturation to become susceptible to deglycosylation by the enzyme. © 1989.

Author-supplied keywords

  • Glycoproteins
  • N-glycosidase
  • Peptide

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Keith J. Mussar

  • Gary J. Murray

  • Brian M. Martin

  • Thammaiah Viswanatha

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free