Polyethyleneimine-coated reticulated vitreous carbon electrode with immobilized enzymes as a substrate detector

  • Tang X
  • Xie B
  • Larsson P
 et al. 
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Abstract

Polyethyleneimine (PEI) was covalently coupled to carbodiimide-activated reticulated vitreous carbon (RVC). The PEI-coated RVC was activated with glutaraldehyde, and glycerol dehydrogenase and diaphorase were then immobilized. The PEI-coated RVC with immobilized enzymes functioned both as an enzyme reactor and a working electrode in an amperometric detection system where NAD+/NADH was recycled by the immobilized enzymes. The coated RVC electrode showed good properties compared to uncoated RVC, such as a long lifetime and a constant response to a series of injections in a flow-injection system, resulting in a relative standard deviation of 1.4%. The calibration graph was linear from the detection limit 0.1μM to 2mM NADH in the absence of recycling. Copyright (C) 1998 Elsevier Science B.V.

Author-supplied keywords

  • Diaphorase
  • Flow injection
  • Glycerol dehydrogenase
  • Polyethyleneimine
  • Reticulated vitreous carbon

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Authors

  • Xiao Jing Tang

  • Per Olof Larsson

  • Bengt Danielsson

  • Masoud Khayyami

  • Gillis Johansson

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