Polyethyleneimine (PEI) was covalently coupled to carbodiimide-activated reticulated vitreous carbon (RVC). The PEI-coated RVC was activated with glutaraldehyde, and glycerol dehydrogenase and diaphorase were then immobilized. The PEI-coated RVC with immobilized enzymes functioned both as an enzyme reactor and a working electrode in an amperometric detection system where NAD+/NADH was recycled by the immobilized enzymes. The coated RVC electrode showed good properties compared to uncoated RVC, such as a long lifetime and a constant response to a series of injections in a flow-injection system, resulting in a relative standard deviation of 1.4%. The calibration graph was linear from the detection limit 0.1μM to 2mM NADH in the absence of recycling. Copyright (C) 1998 Elsevier Science B.V.
Tang, X. J., Xie, B., Larsson, P. O., Danielsson, B., Khayyami, M., & Johansson, G. (1998). Polyethyleneimine-coated reticulated vitreous carbon electrode with immobilized enzymes as a substrate detector. Analytica Chimica Acta, 374(2–3), 185–190. https://doi.org/10.1016/S0003-2670(98)00255-4