Protein distribution amongst chromatin particles following DNAase II digestion

  • Billett M
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Abstract

When active chromatin is released as a Mg-soluble fraction following digestion of nuclei with DNAase II, a concomitant release of HMG proteins, and hnRNP particles occurs. Release of HMG 14 and 17 is dependent on active chromatin release, whereas HMG 1 and 2, and hnRNP particles are released in an independent process. The Mg-soluble fraction comprises a heterogeneous mixture of particles of less compact conformation than normal nucleosomes, and prone to protein-induced aggregation. Histone H1, and HMG 14 and 17 appear to be associated with these particles in a reversible manner, whereas HMG 1 and 2 are unbound. © 1981.

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Authors

  • M. A. Billett

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