Regulation of Pyk2 expression by p56Lck in Jurkat T lymphocytes

  • Franklin R
  • Atherfold P
  • Robinson P
 et al. 
  • 4


    Mendeley users who have this article in their library.
  • 7


    Citations of this article.


Lysates from the Jurkat T lymphocyte cell line were immunoblotted with anti-Pyk2, and two major forms of Pyk2 were identified. When lysates from the p56Lck negative (J.CaM1/Rep3) and CD45 negative Jurkat cell line derivatives were immunoblotted with anti-Pyk2, only the lower mobility form of Pyk2 was predominant. Transfection of J.CaM1 cells with p56Lck restored expression of the multiple forms of Pyk2. Using RT-PCR, we found that both species of the alternatively spliced mRNA for Pyk2 were present in all of the lines regardless of their ability to express CD45 or p56Lck protein. When p56Lck immunoprecipitates were immunoblotted with anti-Pyk2, only the higher mobility form of Pyk2 immunoprecipitated with p56Lck. These data demonstrate that certain members of the Src family of kinases interact preferentially with the different isoforms of Pyk2 and may have a role in the regulation of the Pyk2 protein in lymphocytes. © 2001 Elsevier Science Inc.

Author-supplied keywords

  • CAK-β
  • CD45
  • Pyk2
  • T lymphocytes
  • p56Lck

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Richard A. Franklin

  • Paul A. Atherfold

  • Penni J. Robinson

  • Diane Bonner

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free