Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases

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Abstract

Organophosporus pesticides were determined using a simple biosensor design. Enzymatic membranes prepared by cross-linking with BSA were deposited on a sensor chip consisting of two identical pH-FETs working in the differential mode (ENFET with enzyme, REFET without enzyme). Acetyl- and butyrylcholinesterase membranes were tested using acetyl- and butyrylcholine chloride as substrates. The decrease in the substrate steady-state response caused by exposure of the biosensor to pesticides [diisopropyl fluorophosphate (DFP), trichlorfon, paraoxon-methyl] was used to estimate the enzyme inhibition. The optimized parameters for the measurement procedure were determined and the detection limits were found to be 10-12M for DFP and 10-6M for paraoxon-methyl and trichlorfon. © 1993.

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Hendji, A. M. N., Jaffrezic-Renault, N., Martelet, C., Clechet, P., Shlu’ga, A. A., Strikha, V. I., … Wlodarski, W. B. (1993). Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases. Analytica Chimica Acta, 281(1), 3–11. https://doi.org/10.1016/0003-2670(93)85333-F

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