Oligosaccharides were synthesized from sucrose using the dextransucrase of Leuconostoc mesenteroides, and the products were isolated by chromatography. The oligosaccharide obtained as the main product was identified as leucrose by13C-NMR. Production of leucrose from sucrose was greatly increased when the reaction was done with fructose as an acceptor. The susceptibility of leucrose to carbohydrases was tested, and leucrose was also a useful substrate for detecting or measuring α-glucosidase in enzyme mixtures such as culture filtrates of microorganisms. © 1990.
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