Susceptibility of leucrose to carbohydrases

Abstract

Oligosaccharides were synthesized from sucrose using the dextransucrase of Leuconostoc mesenteroides, and the products were isolated by chromatography. The oligosaccharide obtained as the main product was identified as leucrose by 13C-NMR. Production of leucrose from sucrose was greatly increased when the reaction was done with fructose as an acceptor. The susceptibility of leucrose to carbohydrases was tested, and leucrose was also a useful substrate for detecting or measuring α-glucosidase in enzyme mixtures such as culture filtrates of microorganisms. © 1990.