Tumor-promoting activity of p-hydroxybenzenediazonium is accelerated in Mg-deficient rats

  • Quintero B
  • Planells E
  • Del Carmen Cabeza M
 et al. 
  • 4

    Readers

    Mendeley users who have this article in their library.
  • 3

    Citations

    Citations of this article.

Abstract

Tumor-promoting activity caused by the short-term administration of p-hydroxybenzenediazonium (PDQ) has been assayed in rats fed on a Mg-deficient diet as a reference model versus rats fed on a standard diet as controls. For 5 weeks groups of 20 rats, fed either on the Mg-deficient or standard diet, were treated simultaneously with PDQ. A group of 10 Mg-deficient rats remained untreated. Topical application of PDQ was followed by the appearance of macroscopic alterations in the skin, which were more evident in the Mg-deficient rats. No deaths occurred during the treatment. After 5 weeks' PDQ treatment the rats were killed and histological analyses were made. Tissues from the skin, liver, heart, kidney, lung and thymus were screened by conventional staining methods. Both the PDQ-treated Mg-deficient and PDQ-treated control rats presented tissue lesions, although to a different extent. The untreated Mg-deficient rats showed no such lesions. Mg-deficient rats treated with PDQ developed significant incipient fibrosarcomas (p < 0.05) and extended hyperplasia (p < 0.001), particularly in the skin, accompanied by a significant increase in the thickness of collagen (mean value: 445.4 ± 47.2 μm, p < 0.05) compared to the control PDQ-treated group (mean values: 258.7 ± 36.4 μm). The overall results provide objective proof of tumor-promoting activity after 5 weeks' treatment with PDQ. Such a fast response is interpreted as being linked to the increased vulnerability of the membrane caused by Mg deficiency, which would more readily facilitate the toxic activity of p-hydroxybenzenediazonium ions. © 2005 Elsevier Ireland Ltd. All rights reserved.

Author-supplied keywords

  • Arenediazonium ions
  • Dediazoniation
  • Histological analysis
  • Mg-deficient rats
  • Tumor-promoting activity
  • p-Hydroxybenzenediazonium ion

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free