125I-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells

  • Yagaloff K
  • Hartig P
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Abstract

125I-Lysergic acid diethylamide (125I-LSD) binds with high affinity to serotonergic sites on rat choroid plexus. These sites were localized to choroid plexus epithelial cells by use of a novel high resolution stripping film technique for light microscopic autoradiography. In membrane preparations from rat choroid plexus, the serotonergic site density was 3100 fmol/mg of protein, which is 10-fold higher than the density of any other serotonergic site in brain homogenates. The choroid plexus site exhibits a novel pharmacology that does not match the properties of 5-hydroxytryptamine-1a (5-HT1a), 5-HT1b, or 5-HT2 serotonergic sites. 125I-LSD binding to the choroid plexus site is potently inhibited by mianserin, serotonin, and (+)-LSD. Other serotonergic, dopaminergic, and adrenergic agonists and antagonists exhibit moderate to weak affinities for this site. The rat choroid plexus 125I-LSD binding site appears to represent a new type of serotonergic site which is located on non-neuronal cells in this tissue.

Author-supplied keywords

  • Animals
  • Autoradiography
  • Binding Sites
  • Brain/metabolism
  • Cattle
  • Choroid Plexus/*metabolism
  • Epithelial Cells
  • Epithelium/metabolism
  • Iodine Radioisotopes/diagnostic use
  • Lysergic Acid Diethylamide/*metabolism
  • Mice
  • Rabbits
  • Rats
  • Serotonin/*metabolism
  • Swine

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Authors

  • K A Yagaloff

  • P R Hartig

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