The roots of three varieties of Polygonum cuspidatum were analyzed for resveratrol and its analogs. The powder of the dried roots was extracted with aqueous ethanol (60% v/v) and the extracts obtained were analyzed using RP HPLC with coulometric detection. A simple HPLC method with a multichannel CoulArray detector was developed for the determination of four stilbenes: resveratrol, its glucoside piceid, piceatannol, and its glucoside astringin. Analyses were carried out on a LiChrospher C18 (125 × 4.6 mm id, particle size 5 μm) column with a mobile phase of ammonium acetate (pH 3) and ACN in gradient mode. Four compounds were monitored by a CoulArray electrochemical detector. Potentials of eight electrochemical cells in series were set in the range of 200-900 mV. Optimization of the mobile phase pH was performed. Calibration curves showed good linearity with correlation coefficients (r2) - more than 0.9975. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
CITATION STYLE
Beňová, B., Adam, M., Onderková, K., Královský, J., & Krajíček, M. (2008). Analysis of selected stilbenes in Polygonum cuspidatum by HPLC coupled with CoulArray detection. Journal of Separation Science, 31(13), 2404–2409. https://doi.org/10.1002/jssc.200800119
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