Background - Angiotensin (Ang) II type 2 (AT2) receptor stimulation results in coronary vasodilation in the rat heart. In contrast, AT2 receptor-mediated vasodilation could not be observed in large human coronary arteries. We studied Ang II-induced vasodilation of human coronary microarteries (HCMAs). Methods and Results - HCMAs (diameter, 160 to 500 ‴m) were obtained from 49 heart valve donors (age, 3 to 65 years). Ang II constricted HCMAs, mounted in Mulvany myographs, in a concentration-dependent manner (pEC50, 8.6±0.2; maximal effect [Emax], 79±13% of the contraction to 100 mmol/L K +). The Ang II type 1 receptor antagonist irbesartan prevented this vasoconstriction, whereas the AT2 receptor antagonist PD123319 increased Emax to 97±14% (P<0.05). The increase in E max was larger in older donors (correlation ΔEmax versus age, r=0.47, P<0.05). The PD123319-induced potentiation was not observed in the presence of the NO synthase inhibitor L-NAME, the bradykinin type 2 (B2) receptor antagonist Hoe140, or after removal of the endothelium. Ang II relaxed U46619-preconstricted HCMAs in the presence of irbesartan by maximally 49±16%, and PD123319 prevented this relaxation. Finally, radioligand binding studies and reverse transcription-polymerase chain reaction confirmed the expression of AT2 receptors in HCMAs. Conclusions - AT2 receptor-mediated vasodilation in the human heart appears to be limited to coronary microarteries and is mediated by B 2 receptors and NO. Most likely, AT2 receptors are located on endothelial cells, and their contribution increases with age.
CITATION STYLE
Batenburg, W. W., Garrelds, I. M., Bernasconi, C. C., Juillerat-Jeanneret, L., Van Kats, J. P., Saxena, P. R., & Danser, A. H. J. (2004). Angiotensin II type 2 receptor-mediated vasodilation in human coronary microarteries. Circulation, 109(19), 2296–2301. https://doi.org/10.1161/01.CIR.0000128696.12245.57
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