Antioxidant and membrane effects of procyanidin dimers and trimers isolated from peanut and cocoa

  • Verstraeten S
  • Hammerstone J
  • Keen C
 et al. 
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The antioxidant and membrane effects of dimer (Dim) and trimer (Trim) procyanidins isolated from cocoa (Theobroma cacao) (B- and C-bonded) and peanut (Arachis hypogea L.) skin (A-bonded) were evaluated in phosphatidyl choline liposomes. When liposomes were oxidized with a steady source of oxidants, the above dimers and trimers inhibited to a similar extent lipid oxidation in a concentration (0.33-5 microM)-dependent manner. With respect to membrane effects, Dim A1, Dim B, Trim A, and Trim C increased (Dim A1 = Dim B and Trim A = Trim C), while Dim A2 decreased, membrane surface potential. All of the procyanidins tested decreased membrane fluidity as determined by fluorescent probes at the water-lipid interface, an effect that extended into the hydrophobic region of the bilayer. Both dimers and trimers protected the lipid bilayer from disruption by Triton X-100. The magnitude of the protection was Dim A1 > Dim A2 > Dim B and Trim C > Trim A. Thus, dimers and trimers can interact with membrane phospholipids, presumably with their polar headgroup. As a consequence of this interaction, they can provide protection against the attack of oxidants and other molecules that challenge the integrity of the bilayer.

Author-supplied keywords

  • Antioxidants
  • Arachis hypogea L
  • Flavonoids
  • Lipid oxidation
  • Membrane interactions
  • Polyphenols
  • Procyanidins
  • Theobroma cacao

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  • Sandra V. Verstraeten

  • John F. Hammerstone

  • Carl L. Keen

  • César G. Fraga

  • Patricia I. Oteiza

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