Array comparative genomic hybridization reveals unbalanced gain of the MYCN region in Wilms tumors

  • Schaub R
  • Burger A
  • Bausch D
 et al. 
  • 11


    Mendeley users who have this article in their library.
  • 13


    Citations of this article.


Wilms tumor (WT) is one of the most common solid tumors in childhood. It is characterized by a nonrandom pattern of chromosomal aberrations whose clinical significance is still uncertain. To gain further insight into different genetic events and their corresponding biological role, conventional cytogenetics and array comparative genomic hybridization (array CGH) were performed on 13 tumor samples. In two of these, array CGH revealed, together with other aberrations, a low-level amplification and an unbalanced gain in the region of chromosome bands 2p23∼p24 that encompass the MYCN gene. Both events were confirmed with a MYCN-specific fluorescence in situ hybridization probe, which showed a signal pattern consistent with a small homogenous staining region in one case. In addition, mRNA expression levels for MYCN were determined by quantitative reverse-transcriptase polymerase chain reaction and revealed that gain of chromosomal material was reflected in enhanced levels of MYCN mRNA in both tumors, whereby also additional tumors showed increased MYCN expression. Therefore, our findings suggest that WT is an additional childhood tumor where MYCN gain might play an important role in tumor development. © 2007 Elsevier Inc. All rights reserved.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Beat SchaferUniversity Childrens Hospital Zurich

  • Rahel Schaub

  • Alexa Burger

  • Damaris Bausch

  • Felix K. Niggli

  • David R. Betts

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free