Assessment of in vitro biofilm formation by Helicobacter pylori.

  • Yonezawa H
  • Osaki T
  • Kurata S
 et al. 
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BACKGROUND AND AIMS: Biofilms are surface-bound communities of bacterial cells that are implicated in their survival. As with various bacteria studied to date, Helicobacter pylori can have an alternate lifestyle as a biofilm. We previously reported that strain TK1402 showed a strong biofilm-forming ability in vitro. However, the mechanisms of its biofilm development remain unclear. We analyzed the basic characteristics of the biofilm-forming ability in strain TK1402.

METHODS: In order to characterize the biofilm-forming ability of the H. pylori strains, auto-aggregation, motility and hydrophobicity, which are important factors in biofilm formation by other bacteria, were analyzed. Further, we tested whether cell growth participated in biofilm formation in strain TK1402.

RESULTS: There were no significant differences in the auto-aggregation, motility and hydrophobicity of strain TK1402 compared with the other strains. On the other hand, pre-culture of this strain for 24-48 h resulted in decreased biofilm formation.

CONCLUSION: TK1402 is a strong biofilm-forming strain of H. pylori in Brucella broth supplemented with 7% fetal calf serum. It is possible that biofilm-forming cell growth is a principal factor in biofilm development.

Author-supplied keywords

  • Bacterial Adhesion
  • Biofilms
  • Cell Proliferation
  • Helicobacter Infections
  • Helicobacter Infections: microbiology
  • Helicobacter pylori
  • Helicobacter pylori: growth & development
  • Helicobacter pylori: isolation & purification
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Japan
  • Kinetics

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  • Hideo Yonezawa

  • Takako Osaki

  • Satoshi Kurata

  • Cynthia Zaman

  • Tomoko Hanawa

  • Shigeru Kamiya

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