Cep164, a novel centriole appendage protein required for primary cilium formation

  • Graser S
  • Stierhof Y
  • Lavoie S
 et al. 
  • 197

    Readers

    Mendeley users who have this article in their library.
  • 247

    Citations

    Citations of this article.

Abstract

Primary cilia (PC) function as microtubule-based sensory antennae projecting from the surface of many eukaryotic cells. They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions in PC assembly is derived from the mature centriole, a component of the centrosome. Through a small interfering RNA screen we found several centrosomal proteins (Ceps) to be involved in PC formation. One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail. By immunogold electron microscopy, Cep164 could be localized to the distal appendages of mature centrioles. In contrast to ninein and Cep170, two components of subdistal appendages, Cep164 persisted at centrioles throughout mitosis. Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Susanne Graser

  • York Dieter Stierhof

  • Sébastien B. Lavoie

  • Oliver S. Gassner

  • Stefan Lamla

  • Mikael Le Clech

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free