Characterization of the relA/spoT gene from Bacillus stearothermophilus

  • Wendrich T
  • Beckering C
  • Marahiel M
  • 12


    Mendeley users who have this article in their library.
  • 14


    Citations of this article.


By use of degenerate primers, we amplified a fragment of a relA/spoT homologous gene from Bacillus stearothermophilus. Chromosomal walking enabled us to sequence the entire gene and its flanking regions. The primary sequence of the gene product is 78% identical to the RelA/SpoT homologue of Bacillus subtilis, and both gene loci share a similar genetic organization. The B. stearothermophilus rel gene was analyzed in vivo by heterologous expression in the B. subtilis relA deletion strain TW30, and is shown to complement the growth defects of TW30. The recombinant Rel(Bst) protein was detected by Western immunoanalysis, and synthesizes guanosine-3'-diphosphate-5'- (tri)diphosphate ((p)ppGpp) after amino acid stress and carbon starvation. These in vivo data, the genetic organization, and the primary structure compared to other RelA/SpoT homologues provide circumstantial evidence that the identified gene encodes the only (p)ppGpp synthetase in B. stearothermophilus, presumed to serve also as (p)ppGpp hydrolase. (C) 2000 Federation of European Microbiological Societies.

Author-supplied keywords

  • Bacillus
  • Guanosine pentaphosphate synthetase
  • Guanosine-3'-diphosphate-5'-(tri)diphosphate
  • RelA
  • SpoT
  • Stringent response

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Thomas M. Wendrich

  • Carsten L. Beckering

  • Mohamed A. Marahiel

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free