We test whether coherent control methods based on ultrashort-pulse phase shaping can be applied when the laser light propagates through biological tissue. Our results demonstrate experimentally that the spectral-phase properties of shaped laser pulses optimized to achieve selective two-photon excitation survive as the laser pulses propagate through tissue. This observation is used to obtain functional images based on selective two-photon excitation of a pH-sensitive chromophore in a sample that is placed behind a slice of biological tissue. Our observation of coherent control through scattering tissue suggests possibilities in multiphoton-based imaging and photodynamic therapy.
CITATION STYLE
Dela Cruz, J. M., Pastirk, I., Comstock, M., Lozovoy, V. V., & Dantus, M. (2004). Use of coherent control methods through scattering biological tissue to achieve functional imaging. Proceedings of the National Academy of Sciences of the United States of America, 101(49), 16996–17001. https://doi.org/10.1073/pnas.0407733101
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