Continuous enzymatic synthesis of biodiesel with novozym 435

  • Shaw J
  • Chang S
  • Lin S
 et al. 
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A continuous process for the enzymic synthesis of biodiesel, fatty acid Me esters (FAMEs) from soybean oil, catalyzed by immobilized lipase from Candida antarctica (Novozym 435) was studied. Novozym 435 was packed in a packed-bed reactor and used to catalyze the alcoholysis of methanol and soybean oil to produce FAMEs in a cosolvent system. Response surface methodol. (RSM) and 3-factor-3-level fractional factorial design were employed to evaluate the effects of synthesis parameters, such as reaction temp. (45-65°), flow rate (0.1-0.5 mL/min), and substrate molar ratio of methanol to soybean oil (3:1-5:1) on percentage molar conversion of FAMEs by alcoholysis. Reaction temp. and flow rate had significant effects on the percent molar conversion. From ridge max anal., the optimum conditions for synthesis were as follows: reaction temp. 52°, flow rate 0.1 mL/min, and substrate molar ratio 4.3:1. The predicted value was 74.2%, and the actual exptl. value was 75.2% molar conversion. [on SciFinder(R)]

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  • J. F. Shaw

  • S. W. Chang

  • S. C. Lin

  • T. T. Wu

  • H. Y. Ju

  • C. C. Akoh

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