Abstract
An approach was developed in controlling the expression ratio of two genes encoded in DNA by inserting a terminator sequence between the two genes. Escherichia coli ribosomal RNA operon T1 terminator, or E. coli tryptophane attenuater was introduced between two green fluorescent protein (GFP) mutants: ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence intensity of BFP to GFPuv after transcription and translation reflected the termination efficiency of the transcriptional termination activity of the inserted terminator.
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CITATION STYLE
Nojima, T., Lin, A. C., Yamamoto, T., Endo, I., & Fujii, T. (2006). Controlling the expression ratio of two proteins by inserting a terminator between the two genes. Nucleic Acids Symposium Series (2004), (50), 329–330. https://doi.org/10.1093/nass/nrl164
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