Controlling the expression ratio of two proteins by inserting a terminator between the two genes.

  • Nojima T
  • Lin A
  • Yamamoto T
 et al. 
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Abstract

An approach was developed in controlling the expression ratio of two genes encoded in DNA by inserting a terminator sequence between the two genes. Escherichia coli ribosomal RNA operon T1 terminator, or E. coli tryptophane attenuater was introduced between two green fluorescent protein (GFP) mutants: ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence intensity of BFP to GFPuv after transcription and translation reflected the termination efficiency of the transcriptional termination activity of the inserted terminator.

Author-supplied keywords

  • Escherichia coli
  • Escherichia coli: genetics
  • Gene Expression Regulation
  • Green Fluorescent Proteins
  • Green Fluorescent Proteins: biosynthesis
  • Green Fluorescent Proteins: genetics
  • Luminescent Agents
  • Luminescent Agents: metabolism
  • Protein Biosynthesis
  • Terminator Regions, Genetic
  • Transcription, Genetic

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Authors

  • Takahiko Nojima

  • Angela C Lin

  • Takatoki Yamamoto

  • Isao Endo

  • Teruo Fujii

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