Crystal structure of human enterovirus 71 3C protease

  • Cui S
  • Wang J
  • Fan T
 et al. 
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Abstract

Human enterovirus 71 (EV71) is the major pathogen that causes hand, foot and mouth disease that particularly affects young children. Growing hand, foot and mouth disease outbreaks were observed worldwide in recent years and caused devastating losses both economically and politically. However, vaccines or effective drugs are unavailable to date. The genome of EV71 consists of a positive sense, single-stranded RNA of ∼ 7400 bp, encoding a large precursor polyprotein that requires proteolytic processing to generate mature viral proteins. The proteolytic processing mainly depends on EV71 3C protease (3C pro) that possesses both proteolysis and RNA binding activities, which enable the protease to perform multiple tasks in viral replication and pathogen-host interactions. The central roles played by EV71 3Cpro make it an appealing target for antiviral drug development. We determined the first crystal structure of EV71 3Cpro and analyzed its enzymatic activity. The crystal structure shows that EV71 3Cpro has a typical chymotrypsin-like fold that is common in picornaviral 3Cpro. Strikingly, we found an important surface loop, also denoted as β-ribbon, which adopts a novel open conformation in EV71 3Cpro. We identified two important residues located at the base of the β-ribbon, Gly123 and His133, which form hinges that govern the intrinsic flexibility of the ribbon. Structure-guided mutagenesis studies revealed that the hinge residues are important to EV71 3Cpro proteolytic activities. In summary, our work provides the first structural insight into EV71 3Cpro, including a mobile β-ribbon, which is relevant to the proteolytic mechanism. Our data also provides a framework for structure-guided inhibitor design against EV71 3Cpro. © 2011 Elsevier Ltd.

Author-supplied keywords

  • 3C pro
  • 3C protease
  • ASU
  • Amino Acid
  • Amino Acid Sequence
  • CVB
  • Crystallography
  • Cysteine Endopeptidases
  • DNA
  • EV71
  • Enterovirus 71
  • Enterovirus 71 3c proteanase
  • Enterovirus A
  • Escherichia coli
  • FMDV
  • Foot-and-mouth disease virus
  • HAV
  • HFMD
  • HRV
  • Hepatitis A virus
  • Human
  • Human enterovirus 71
  • Human enterovirus B
  • Human rhinovirus sp.
  • Humans
  • Models
  • Molecular
  • Molecular Sequence Data
  • Mutagenesis
  • PDB
  • PV
  • Picornavirus
  • Poliovirus
  • Protein Data Bank
  • Protein Structure
  • RNA
  • RNA binding
  • SARS coronavirus
  • SARS-CoV
  • SLS
  • Sequence Analysis
  • Sequence Homology
  • Site-Directed
  • Swiss Light Source
  • Tertiary
  • Viral
  • Viral Proteins
  • WT
  • X-Ray
  • antiviral activity
  • antivirus agent
  • article
  • asymmetric unit
  • chymotrypsin
  • coxsackievirus B
  • crystal structure
  • enzyme activity
  • foot and mouth disease
  • foot-and-mouth disease virus
  • hand
  • hand foot and mouth disease
  • hepatitis A virus
  • host pathogen interaction
  • human enterovirus 71
  • human rhinovirus
  • mutagenesis
  • poliovirus
  • priority journal
  • protein degradation
  • proteinase
  • severe acute respiratory syndrome-coronavirus
  • single stranded RNA
  • structure analysis
  • unclassified drug
  • virus enzyme
  • virus replication
  • wild-type

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Authors

  • S Cui

  • J Wang

  • T Fan

  • B Qin

  • L Guo

  • X Lei

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