Decellularization does not eliminate thrombogenicity and inflammatory stimulation in tissue-engineered porcine heart valves.

  • Kasimir M
  • Rieder E
  • Seebacher G
 et al. 
  • 51


    Mendeley users who have this article in their library.
  • 101


    Citations of this article.


BACKGROUND AND AIM OF THE STUDY In tissue engineering of heart valves using decellularized xenogenic valves, it has been suggested that cell elimination would result in a biologically inert matrix. The aim of this in-vitro investigation was to evaluate different decellularization methods in regard to the completeness of cell removal, inflammatory response, and thrombocyte activation. METHODS Decellularized porcine Synergraft valves were compared with porcine pulmonary conduits decellularized with Triton X-100, sodium deoxycholate, Igepal CA-630 and ribonuclease. Completeness of decellularization was evaluated with staining for nuclei and alpha-Gal epitope. Decellularized heart valves with and without seeding with endothelial cells (ECs) were incubated with human platelet-rich plasma and stained for CD41 and PAC-1 to evaluate thrombocyte activation. Samples were processed for laser scanning microscopy (LSM) and scanning electron microscopy (SEM). Migration of human monocytic cells towards extracted valve proteins was tested. RESULTS In contrast to the Synergraft, complete cell removal and elimination of the alpha-gal epitope was achieved with the new decellularization method. Numerous adherent and activated platelets were found on the decellularized matrix. This was inhibited by seeding with ECs. Even in completely cell-free valve tissue extracellular matrix proteins attracted human monocytic cells as in early inflammation, depending on whether porcine or human tissue was used. CONCLUSION Important differences were found in the decellularization efficacy of treatment methods. However, even complete elimination of cells and their remnants did not result in a biologically inert matrix. The decellularized porcine heart valve matrix has the potential to attract inflammatory cells and to induce platelet activation. These findings suggest that it will be important to control the different inflammation-stimulating factors if porcine tissues are to be used successfully in tissue engineering.

Author-supplied keywords

  • Animal
  • Animals
  • Cell Movement
  • Cell Movement: drug effects
  • Confocal
  • Deoxycholic Acid
  • Deoxycholic Acid: pharmacology
  • Detergents
  • Detergents: pharmacology
  • Disease Models
  • Heart Valve Diseases
  • Heart Valve Diseases: pathology
  • Heart Valve Diseases: surgery
  • Heart Valve Prosthesis
  • Heart Valve Prosthesis Implantation
  • Heart Valve Prosthesis Implantation: adverse effec
  • Microscopy
  • Octoxynol
  • Octoxynol: pharmacology
  • Platelet Activation
  • Platelet Activation: drug effects
  • Polyethylene Glycols
  • Polyethylene Glycols: pharmacology
  • Postoperative Complications
  • Postoperative Complications: etiology
  • Postoperative Complications: prevention & control
  • Prosthesis Design
  • Pulmonary Valve
  • Pulmonary Valve: drug effects
  • Pulmonary Valve: surgery
  • Pulmonary Valve: ultrastructure
  • Ribonucleases
  • Ribonucleases: pharmacology
  • Swine
  • Thrombosis
  • Thrombosis: etiology
  • Thrombosis: prevention & control
  • Tissue Embedding
  • Tissue Embedding: methods

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Marie-Theres Kasimir

  • Erwin Rieder

  • Gernot Seebacher

  • Anneliese Nigisch

  • Barbara Dekan

  • Ernst Wolner

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free