Design and application of a novel ionic liquid with the property of strengthening coenzyme regeneration for whole-cell bioreduction in an ionic liquid-distilled water medium

  • Li J
  • Wang P
  • Huang J
 et al. 
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Focusing on the task-specific properties of ionic liquids (ILs), a novel IL was designed by combining the quaternary ammonium cationic surfactant, [N1,1,1,1]+, with benign amino acid anion ([Cys]-), and was successfully employed in whole-cell-catalyzed bioreduction by Trichoderma asperellum ZJPH0810 using an ionic liquid-distilled water medium. As expected, based on better understanding about the effects of ILs' characteristics of cations and anions on T. asperellum ZJPH0810-catalyzed bioreduction and the optimization of reaction parameters, the developed tetramethylammonium cysteine ([N1,1,1,1][Cys])-containing system is more efficient for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone to (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by whole-cell catalysis in contrast to that in monophasic aqueous system. Herein, a new biotechnological application for designed type of ILs is proposed due to its novel property of strengthening coenzyme regeneration during the bioreduction process. The designed IL [N1,1,1,1][Cys] was also evaluated in Candida tropicalis 104-catalyzed bioreduction to board its application, with remarkable success.

Author-supplied keywords

  • Asymmetric biocatalytic reduction
  • Chiral alcohol
  • Coenzyme regeneration
  • Tetramethylammonium cysteine ([N1,1,1,1][Cys])-containing system

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  • Jun Li

  • Pu Wang

  • Jin Huang

  • Jia Sun

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