A highly sensitive method for determination of berberine is proposed based on the measurements of total internal reflected fluorescence (TIRF) at water/ tetrachloromethane (H2O/CCl4) interface. In the pH range of 2.6-5.7, the co-adsorption of the berberine with the anionic surfactants such as sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDS), and sodium lauryl sulfate (SLS) occurs at the H2O/CCl4 interface, resulting in greatly enhanced TIRF signal characterized by the emission at 526 nm when excited with a 351 nm light beam. The enhanced TIRF intensity is in proportion to the berberine concentration in the range 0.2-10.0×10-7 mol L-1. The limit of detection is 1.7×10-9 mol L-1 (3σ). It was found that ions such as Ca(II), Cu(II), Fe(III), Cd(II), Mg(II), Zn(II), Pb(II), and Al(III) can be allowed larger than 1.0×10-4 mol L-1. Meanwhile, the organic compounds such as vitamin B, saccharine, and amino acid do not display any effect for the present TIRF method even if they are larger than 1.0×10-2mol L-1in high concentration levels (larger than 1.0×10-5 mol L-1). The results of determination for synthetic samples were agreement with the desired values, and the ones for tablets were identical with those obtained according to the method of Chinese Pharmacopoeia. © Springer-Verlag 2003.
CITATION STYLE
Feng, P., Huang, C. Z., & Li, Y. F. (2003). Determination of berberine by measuring the enhanced total internal reflected fluorescence at water/tetrachloromethane interface in the presence of sodium dodecyl benzene sulfonate. Analytical and Bioanalytical Chemistry, 376(6), 868–872. https://doi.org/10.1007/s00216-003-1967-1
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