The effects of ozone and oxygen on the degradation of carotenoids in an aqueous model system were studied. All-trans β-carotene, 9-cis β-carotene, β-cryptoxanthin, and lycopene were adsorbed onto a C18 solid phase and exposed to a continuous flow of water saturated with oxygen or ozone at 30 °C. Carotenoids were analyzed using HPLC with a C30 column and a photodiode array detector. Approximately 90% of all-trans β-carotene, 9-cis β-carotene, and β-cryptoxanthin were lost after exposure to ozone for 7 h. A similar loss of lycopene occurred in only 1 h. When exposed to oxygen, all carotenoids, except β-cryptoxanthin, degraded at lower rates. The degradation of all the carotenoids followed zero-order reaction kinetics with the following relative rates: lycopene > β-cryptoxanthin > all-trans β-carotene > 9-cis β-carotene. The major degradation products of β-carotene were tentatively identified on the basis of their elution on the HPLC column, UV - Vis spectra, and electrospray LC - MS. Predominant isomers of β-carotene were 13-cis, 9-cis, and a dicis isomer. Products resulting from cleavage of the molecule were β-apo-13-carotenone and β-apo-14'-carotenal, whereas epoxidation yielded β-carotene 5,8-epoxide and β-carotene 5,8-endoperoxide.
CITATION STYLE
Henry, L. K., Puspitasari-Nienaber, N. L., Jaren-Galan, M., Van Breemen, R. B., Catignani, G. L., & Schwartz, S. J. (2000). Effects of ozone and oxygen on the degradation of carotenoids in an aqueous model system. Journal of Agricultural and Food Chemistry, 48(10), 5008–5013. https://doi.org/10.1021/jf000503o
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