Efficient Differentiation of Human Embryonic Stem Cells into Oligodendrocyte Progenitors for Application in a Rat Contusion Model of Spinal Cord Injury

  • Kerr C
  • Letzen B
  • Hill C
 et al. 
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Abstract

ABSTRACT This study utilized a contusion model of spinal cord injury (SCI) in rats using the standardized NYU-MASCIS impactor, after which oligodendrocyte progenitor cells (OPCs) derived from human embryonic stem cell (ESC) were transplanted into the spinal cord to study their survival and migration route toward the areas of injury. One critical aspect of successful cell-based SCI therapy is the time of injection following injury. OPCs were injected at two clinically relevant times when most damage occurs to the surrounding tissue, 3 and 24 hours following injury. Migration and survivability after eight days was measured postmortem. In-vitro immunofluorescence revealed that most ESC-derived OPCs expressed oligodendrocyte markers, including CNPase, GalC, Olig1, O4, and O1. Results showed that OPCs survived when injected at the center of injury and migrated away from the injection sites after one week. Histological sections revealed integration of ESC-derived OPCs into the spinal cord with contusion injury ...

Author-supplied keywords

  • contusion
  • embryonic stem cells
  • motor behavior
  • oligodendrocyte
  • somatosensory evoked potential
  • spinal cord injury

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Authors

  • Candace L. Kerr

  • Brian S. Letzen

  • Christine M. Hill

  • Gracee Agrawal

  • Nitish V. Thakor

  • Jared L. Sterneckert

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