The electrical response of cerebellar Purkinje neurons to simulated ischaemia

  • Hamann M
  • Rossi D
  • Mohr C
 et al. 
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Abstract

Despite lacking N-methyl-D-aspartate receptors, cerebellar Purkinje cells are highly vulnerable to ischaemic insults, which lead them to die necrotically in an -amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor-dependent manner. To investigate the electrical events leading to this cell death, we whole-cell clamped Purkinje cells in cerebellar slices. Simulated ischaemia evoked an initial hyperpolarization of Purkinje cells by 8.5 mV, followed by a regenerative 'anoxic depolarization' (AD) to -14 mV. The AD was prevented by glutamate receptor blockers. In voltage-clamp mode, we used the cells' glutamate receptors to sense the rise of extracellular glutamate concentration induced by ischaemia, with GABA(A) and GABA(B) receptors blocked and Cs+ as the main pipette cation. Ischaemia induced a small (

Author-supplied keywords

  • Anoxia
  • Cerebellum
  • Exocytosis
  • Glutamate transporter
  • Ischaemia

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Authors

  • Martine Hamann

  • David J. Rossi

  • Claudia Mohr

  • Adriana L. Andrade

  • David Attwell

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