Endogenous isoprenoid and aromatic cytokinins in different plant parts of Cocos nucifera (L.)

  • Sáenz L
  • Jones L
  • Oropeza C
 et al. 
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The present study reports the analyses of both isoprenoid and aromatic
cytokinins in the coconut palm by combined high performance liquid
chromatography and group specific enzyme immunoassays ( HPLC- ELISA).
The results showed that the isoprenoid cytokinins were several fold more
abundant than the aromatic cytokinins in each of the plant parts
analysed: immature inflorescence, shoot apical meristem ( SAM), spear
leaf and embryo. Within the isoprenoid cytokinins, the most abundant
ones by type were the zeatin- ( Z-), the isopentenyladenine( iP-) and
the dihydrozeatin- ( DHZ-) type in decreasing order for most plant parts
studied, and individually, zeatin riboside ( ZR) or zeatin riboside- 5
`- monophosphate ( ZR5' P) depending on the part. In the case of the iP-
type cytokinins, the results showed that its 9- glucoside was the most
abundant one in most parts. The isoprenoid cytokinin profiles in coconut
showed a predominant pattern of 9- conjugation as a major metabolism
route for these cytokinins. Analyses also showed the occurrence of the
aromatic cytokinin 6- benzylaminopurine ( BAP) and its riboside ( BAPR),
9- glucoside ( BAP9G), and nucleotide ( BAPR5' P). Their presence in
coconut palm was unequivocally identified after permethylation by gas
chromatography- mass spectrometry. They were more concentrated in the
embryo and in the immature inflorescence than in the other two parts
studied, however their concentration in each part was several times
lower than that of isoprenoid cytokinins. All four were detected in each
of the parts studied. The most abundant ones were BAPR and BAP9G in
immature inflorescence; and BAPR in all of the other parts. When all
cytokinins analysed are considered, differences between the plant parts
studied were found. The zygotic embryos showed the highest content,
double that in immature inflorescence, and five times more that in spear
leaf and SAM. These differences are even greater when individual
cytokinins are compared.

Author-supplied keywords

  • Cocos nucifera
  • Cytokinin identification
  • Cytokinin quantification
  • GC-MS

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  • L. Sáenz

  • L. H. Jones

  • C. Oropeza

  • D. Vláčil

  • M. Strnad

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