Entamoeba histolytica lacks trypanothione metabolism

  • Ariyanayagam M
  • Fairlamb A
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Abstract

Entamoeba histolytica lacks glutathione reductase activity and the ability to synthesise glutathione de novo. However, a recent report suggested that exogenous glutathione can be taken up and conjugated to spermidine to form trypanothione, a metabolite found so far only in trypanosomatids. Given the therapeutic implications of this observation, we have carefully analysed E. histolytica for evidence of trypanothione metabolism. Using a sensitive fluorescence-based HPLC detection system we could confirm previous reports that cysteine and hydrogen sulphide are the principal low molecular mass thiols. However, we were unable to detect trypanothione or its precursor N1-glutathionylspermidine [

Author-supplied keywords

  • Entamoeba histolytica
  • Evolution
  • Glutathione
  • Mitochondrion
  • Thiols
  • Trypanothione

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