Ethanol increases astrocyte aquaporin-4 expression under hyper-sodium condition.

  • Katada R
  • Sugimoto K
  • Yoshida M
 et al. 
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Ethanol increases brain aquaporin-4 (AQP4) expression after traumatic brain injury (TBI), leading to augment mortality and morbidity after TBI. AQP4 is regulated by sodium ion channels/transporters. Ethanol affects the ion channels/transporters. From these findings, we hypothesized that ethanol may have different effects on AQP4 expression in hypo- or hyper-sodium condition. In this study, rat primary astrocytes were incubated in iso-, hypo- or hyper-sodium MEM medium with 10% calf serum. Ethanol was added to each medium simultaneously. And to check whether hypo/hyper-sodium condition could change AQP4 expression after ethanol exposure or not, astrocytes were incubated in iso-sodium with ethanol, followed by changed to hypo/hyper-sodium with the same concentration of ethanol. Astrocyte AQP4 expression was increased in hypo-sodium exposure. Hypo-sodium with ethanol did not change AQP4 expression significantly, on the other hand, hyper-sodium with ethanol decreased AQP4 expression for short time exposure, and increased it for long time exposure. Hyper-sodium changing increased astrocyte AQP4 expression under EtOH exposure. These findings suggest that AQP4 expression is regulated by sodium ion or ion channels/transporters. And ethanol affects sodium ion channels/transporters, which is involved in AQP4 expression under ethanol.

Author-supplied keywords

  • Animals
  • Aquaporin 4
  • Aquaporin 4: genetics
  • Aquaporin 4: metabolism
  • Astrocytes
  • Astrocytes: metabolism
  • Brain Injuries
  • Brain Injuries: genetics
  • Brain Injuries: metabolism
  • Cells, Cultured
  • Culture Media
  • Culture Media: chemistry
  • Dose-Response Relationship, Drug
  • Ethanol
  • Ethanol: adverse effects
  • Gene Expression
  • Gene Expression: drug effects
  • Hypernatremia
  • Hypernatremia: genetics
  • Hypernatremia: metabolism
  • Rats
  • Rats, Wistar
  • Sodium
  • Sodium Channels
  • Sodium Channels: drug effects
  • Sodium Channels: physiology
  • Sodium: adverse effects
  • Sodium: analysis
  • Sodium: physiology

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  • SCOPUS: 2-s2.0-84907880278
  • ISSN: 1341-8963
  • PMID: 25223087
  • SGR: 84907880278
  • PUI: 373973373


  • Ryuichi Katada

  • Kana Sugimoto

  • Motonori Yoshida

  • Hiroshi Matsumoto

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