Evaluation of biological sample preparation for immunosignature-based diagnostics.

  • Chase B
  • Johnston S
  • Legutki J
  • 7

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Abstract

To address the need for a universal system to assess health status, we previously described a method termed "immunosignaturing" which splays the entire humoral antibody repertoire across a peptide microarray. Two important issues relative to the potential broad use of immunosignatures are sample preparation and stability. In the present study, we compared the immunosignatures developed from serum, plasma, saliva, and antibodies eluted from blood dried onto filter paper. We found that serum and plasma provide identical immunosignatures. Immunosignatures derived from dried blood also correlated well with those from nondried serum from the same individual. Immunosignatures derived from dried blood were capable of distinguishing naïve mice from those infected with influenza virus. Saliva was applied to the arrays, and the IgA immunosignature correlated strongly with that from dried blood. Finally, we demonstrate that dried blood retains immunosignature information even when exposed to high temperature. This work expands the potential diagnostic uses for immunosignatures. These features suggest that different forms of archival samples can be used for diagnosis development and that in prospective studies samples can be easily procured.

Author-supplied keywords

  • Animal
  • Animals
  • Antibodies
  • Antibodies: analysis
  • Blood
  • Blood: immunology
  • Clinical Laboratory Techniques
  • Clinical Laboratory Techniques: methods
  • Disease Models
  • Female
  • Inbred BALB C
  • Mice
  • Microarray Analysis
  • Microarray Analysis: methods
  • Orthomyxoviridae Infections
  • Orthomyxoviridae Infections: diagnosis
  • Orthomyxoviridae Infections: immunology
  • Plasma
  • Plasma: immunology
  • Protein Array Analysis
  • Protein Array Analysis: methods
  • Saliva
  • Saliva: immunology
  • Serum
  • Serum: immunology
  • Specimen Handling
  • Specimen Handling: methods

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Authors

  • Brian Andrew Chase

  • Stephen Albert Johnston

  • Joseph Barten Legutki

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