Fluorescence recovery after photobleaching.

  • Carisey A
  • Stroud M
  • Tsang R
 et al. 
  • 15

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Abstract

This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.

Author-supplied keywords

  • Algorithms
  • Animals
  • Cell Adhesion Molecules
  • Cell Adhesion Molecules: chemistry
  • Cell Adhesion Molecules: metabolism
  • Cell Culture Techniques
  • Fluorescence Recovery After Photobleaching
  • Fluorescence Recovery After Photobleaching: instru
  • Fluorescence Recovery After Photobleaching: method
  • Focal Adhesions
  • Focal Adhesions: chemistry
  • Focal Adhesions: metabolism
  • Green Fluorescent Proteins
  • Green Fluorescent Proteins: chemistry
  • Green Fluorescent Proteins: metabolism
  • Mice
  • Microscopy, Fluorescence
  • NIH 3T3 Cells
  • Recombinant Fusion Proteins
  • Recombinant Fusion Proteins: chemistry
  • Recombinant Fusion Proteins: metabolism
  • Software
  • Time-Lapse Imaging
  • Time-Lapse Imaging: instrumentation
  • Time-Lapse Imaging: methods

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Authors

  • Alex Carisey

  • Matthew Stroud

  • Ricky Tsang

  • Christoph Ballestrem

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