Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3

  • Kumagai S
  • Suzuki T
  • Tezuka K
 et al. 
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Abstract

Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Author-supplied keywords

  • C-terminal domain
  • Cadmium
  • OsHMA3
  • P1B-ATPase (HMA)
  • Rice
  • Vacuolar sequestration

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Authors

  • Saori Kumagai

  • Tatsuya Suzuki

  • Kouichi Tezuka

  • Namiko Satoh-Nagasawa

  • Hidekazu Takahashi

  • Kenji Sakurai

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