Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3

Abstract

Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P 1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.