Functions of a GnRH receptor heterodimer of the ascidian, Ciona intestinalis.

  • Sakai T
  • Aoyama M
  • Kusakabe T
 et al. 
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Abstract

Gonadotropin-releasing hormone (GnRH) is a ten-amino acid peptide hormone that plays pivotal roles in reproduction in vertebrates and octopus. Recently, six GnRH forms (t-GnRH-3-8) and four GnRH receptor subtypes (Ci-GnRHR-1-4) were identified in the protochordate, Ciona intestinalis. In this study, we show the functional modulation of Ci-GnRHR-1 via heterodimerization with the orphan receptor subtype, Ci-GnRHR-4. The dimerization between Ci-GnRHR-1 and R-4 was detected by co-immunoprecipitation and immunoblot analysis. Binding assays confirmed the binding of t-GnRHs to Ci-GnRHR-1 but not to R-4, and verified no alternation in ligand-binding affinity between Ci-GnRHR-1 homodimer and Ci-GnRHRI&4 heterodimer. The heterodimer was found to stimulate the elevation of intracellular calcium, time-extension of ERK phosphorylation, and up-regulation of cell proliferation, all in a ligand specific manner, compared with the Ci-GnRHR-1 homodimer. In combination, these results indicated that Ci-GnRHR-4 is not an inactive receptor, but a modulatory factor for Ci-GnRHR-1 in C. intestinalis.

Author-supplied keywords

  • Animals
  • Calcium
  • Calcium: metabolism
  • Cell Proliferation
  • Ciona intestinalis
  • Ciona intestinalis: cytology
  • Ciona intestinalis: metabolism
  • Dimerization
  • Gonadotropin-Releasing Hormone
  • Gonadotropin-Releasing Hormone: chemistry
  • Gonadotropin-Releasing Hormone: metabolism
  • MAP Kinase Signaling System
  • Models, Biological
  • Protein Structure, Quaternary
  • Receptors, LHRH
  • Receptors, LHRH: chemistry
  • Receptors, LHRH: classification
  • Receptors, LHRH: metabolism
  • Signal Transduction

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Authors

  • T Sakai

  • M Aoyama

  • T Kusakabe

  • M Tsuda

  • H Satake

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