Gene transfer to arteries.

  • Akyürek L
  • San H
  • Nabel E
 et al. 
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Abstract

This unit provides a set of protocols for introducing recombinant genes into normal, injured, and atherosclerotic arteries. The protocols include animal preparation, surgical techniques, and delivery systems. Protocols describe gene delivery to normal, injured, and stented porcine iliofemoral arteries, employing a double balloon infusion catheter to deliver the vector. Another basic protocol describes gene delivery to atherosclerotic arteries using a hyperlipidemic double-injury rabbit model, and requires surgical exposure of the artery and instillation of the gene vector via a catheter. Additional protocols describe gene delivery to normal and injured murine carotid and femoral arteries. An Alternate Protocol describes a percutaneous method for arterial gene delivery. These protocols may be adapted to deliver genes to either injured or noninjured atherosclerotic arteries.

Author-supplied keywords

  • Animals
  • Arteries
  • Arteries: metabolism
  • Atherosclerosis
  • Atherosclerosis: genetics
  • Atherosclerosis: therapy
  • Carotid Arteries
  • Carotid Arteries: metabolism
  • Disease Models, Animal
  • Femoral Artery
  • Femoral Artery: injuries
  • Femoral Artery: metabolism
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Vectors
  • Genetics, Medical
  • Humans
  • Iliac Artery
  • Iliac Artery: metabolism
  • Mice
  • Rabbits
  • Swine

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Authors

  • Levent M Akyürek

  • Hong San

  • Elizabeth G Nabel

  • Ripudamanjit Singh

  • Robert D Simari

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