Grease matrix as a versatile carrier of proteins for serial crystallography

  • Sugahara M
  • Mizohata E
  • Nango E
 et al. 
  • 86


    Mendeley users who have this article in their library.
  • 73


    Citations of this article.


Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Michihiro Sugahara

  • Eiichi Mizohata

  • Eriko Nango

  • Mamoru Suzuki

  • Tomoyuki Tanaka

  • Tetsuya Masuda

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free