Identification of the Plasmodium vivax mdr-like gene (pvmdr1) and analysis of single-nucleotide polymorphisms among isolates from different areas of endemicity.

  • Brega S
  • Meslin B
  • de Monbrison F
 et al. 
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Abstract

Because of the lack of methods for continuous in vitro culture of Plasmodium vivax, little is known about drug-resistance mechanisms in this malaria-causing parasite. Therefore, identification of all the genes potentially involved in drug resistance and of molecular markers related to drug resistance would provide a framework for studying the incidence and spread of drug-resistant P. vivax strains. We have identified the P. vivax orthologue of the pfmdr1 gene (pvmdr1), which was shown to have a role in the drug resistance of Plasmodium falciparum. Comparison of the alignments of both nucleotide and amino acid sequences of pvmdr1 with those of other Plasmodium multidrug-resistance genes revealed an open-reading frame of 4392 base pairs encoding a deduced protein of 1464 amino acids. Nucleotide polymorphisms at 2 codons of the pvmdr1 gene--Y976F and F1076L--were found in 14 of 23 P. vivax isolates from different areas of endemicity, including Thailand, Indonesia, Turkey, Azerbaijan, and French Guyana.

Author-supplied keywords

  • Amino Acid Sequence
  • Animals
  • Drug Resistance
  • Drug Resistance: genetics
  • Endemic Diseases
  • Humans
  • Molecular Sequence Data
  • P-Glycoprotein
  • P-Glycoprotein: genetics
  • Plasmodium vivax
  • Plasmodium vivax: genetics
  • Polymorphism
  • Single Nucleotide

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Authors

  • Sara Brega

  • Benoit Meslin

  • Frédérique de Monbrison

  • Carlo Severini

  • Luigi Gradoni

  • Rachanee Udomsangpetch

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