Identification of a Renibacterium salmoninarum DNA fragment associated with bacterial internalization into CHSE-cultured cells

  • Maulén N
  • Morales P
  • Aruti D
 et al. 
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We report here the isolation of a Renibacterium salmoninarum DNA sequence capable of transforming a non-invasive Escherichia coli strain into a microorganism able to enter the fish cell line, CHSE-214. Immunofluorescence and electron microscopy techniques were used to assess the acquired invasive phenotype by HB101 E. coli cells, upon transformation with pPMV-189. This plasmid carries a 2282-bp R. salmoninarum DNA segment. The invasive phenotype is conserved upon deletion of approximately 1000 bp at the 3' end of the insert. The remaining segment contains an ORF region encoding a putative protein of about 30 kDa.

Author-supplied keywords

  • Bacterial internalization
  • Bacterial kidney disease
  • Renibacterium salmoninarum

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  • Nancy P. Maulén

  • Pedro J. Morales

  • Dora Aruti

  • Jaime E. Figueroa

  • Margarita I. Concha

  • Manuel Krauskopf

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