Immobilization of oligonucleotides onto a glass support via disulfide bonds: A method for preparation of DNA microarrays

  • Rogers Y
  • Jiang-Baucom P
  • Huang Z
 et al. 
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The covalent attachment of disulfide-modified oligonucleotides to a mercaptosilane-modified glass surface is described. This method provides an efficient and specific covalent attachment chemistry for immobilization of DNA probes onto a solid support. Glass slides were derivatized with 3- mercaptopropyl silane for attachment of 5-prime disulfide-modified oligonucleotides via disulfide bonds. An attachment density of approximately 3 x 105oligonucleotides/μm2was observed. Oligonucleotides attached by this method provided a highly efficient substrate for nucleic acid hybridization and primer extension assays. In addition, we have demonstrated patterning of multiple DNA probes on a glass surface utilizing this attachment chemistry, which allows for array densities of at least 20,000 spots/cm2.

Author-supplied keywords

  • Covalent immobilization
  • DNA microarray
  • Disulfide bonds
  • Glass
  • Oligonucleotide

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  • Yu Hui Rogers

  • Ping Jiang-Baucom

  • Zhi Jian Huang

  • Valery Bogdanov

  • Stephen Anderson

  • Michael T. Boyce-Jacino

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