Intestinal scavenger receptors are involved in vitamin K1 absorption

  • Goncalves A
  • Margier M
  • Roi S
 et al. 
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Vitamin K1 (phylloquinone) intestinal absorption is thought to be mediated by a carrier protein that still remains to be identified. Apical transport of vitamin K1 was examined using Caco-2 TC-7 cell monolayers as a model of human intestinal epithelium and in transfected HEK cells. Phylloquinone uptake was then measured ex vivo using mouse intestinal explants. Finally, vitamin K1 absorption was compared between wild-type mice and mice overexpressing SR-BI in the intestine and mice deficient in CD36. Phylloquinone uptake by Caco-2 cells was saturable and was significantly impaired by co-incubation with α-tocopherol (and vice versa). Anti-human SR-BI antibodies and BLT1 (a chemical inhibitor of lipid transport via SR-BI) blocked up to 90% of vitamin K1 uptake. BLT1 also decreased phylloquinone apical efflux by approximately 80%. Transfection of HEK cells with SR-BI and CD36 significantly enhanced vitamin K1 uptake, which was subsequently decreased by the addition of BLT1 or SSO (CD36 inhibitor), respectively. Similar results were obtained in mouse intestinal explants. In vivo, the phylloquinone postprandial response was significantly higher, and the proximal intestine mucosa phylloquinone content 4 h after gavage was increased in mice overexpressing SR-BI compared to controls. Phylloquinone postprandial response was also significantly increased in CD36-deficient mice compared to wild-type mice, but their vitamin K1 intestinal content remained unchanged. Overall, the present data demonstrate for the first time that intestinal scavenger receptors participate to the absorption of dietary phylloquinone.

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  • A Goncalves

  • M Margier

  • S Roi

  • X Collet

  • I Niot

  • P Goupy

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