Purpose: Since isoflurane is known to attenuate endothelium-dependent dilation (EDD) in normal cerebral arterioles, we examined whether the anesthetic has a similar effect and further impairs EDD in vessels exposed to SAH. Methods: Autologous blood was introduced in the subarachnoid space and the parietal lobe harvested. Control animals were sacrificed without introduction of blood. The response of microvessles to the endothelium-dependent dilator adenosine diphosphate (ADP) 10-9-10-4 M, the endothelium-independent dilator nitroprusside 10-9-10-4 M, and ET-1 10-13-10-8 M was measured by videomicroscopy in the presence of 0-2 minimum alveolar concentration (MAC) of isoflurane. Results: Isoflurane attenuated EDD to ADP in control vessels [66 ± 5% (control) vs 27 ± 11% (2 MAC) dilation to ADP 10-4 M, P < 0.05]. Although SAH was associated with reduced dilation to ADP, exposure to isoflurane did not further impair dilation to ADP after SAH [26 ± 3% (SAH) vs 21 ± 5% (SAH/2 MAC) dilation to ADP 10-4 M, P = NS]. Dilation to nitroprusside was not affected by isoflurane or SAH. Constriction to ET-1 was reduced by 2 MAC of isoflurane [21 ± 1% (control) vs 13 ± 5% (2 MAC) constriction to ET-1 10-8 M, P < 0.05], but not by 1 MAC of isoflurane in control vessels. Constriction to ET-1 was greatly attenuated by 1 or 2 MAC of isoflurane after SAH [32 ± 5% (SAH) vs 18 ± 4% (SAH/2 MAC) constriction to ET-1 10-8 M, P < 0.05]. Conclusion: In rats, isoflurane does not further impair EDD after SAH and modulates the constrictive response to ET-1. Such an effect of isoflurane would not predispose the SAH-exposed vessels to vasospasm.
CITATION STYLE
Park, K. W., Dai, H. B., Metais, C., Comunale, M. E., & Sellke, F. W. (2002). Isoflurane does not further impair microvascular vasomotion in a rat model of subarachnoid hemorrhage. Canadian Journal of Anesthesia, 49(4), 427–433. https://doi.org/10.1007/BF03017335
Mendeley helps you to discover research relevant for your work.