Isolation and characterization of novel EST‐derived genic markers in Pisum sativum (Fabaceae)

Abstract

• Premise of the study: Novel markers were developed for pea ( Pisum sativum ) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross‐species transferability. • Methods and Results: Seventy‐seven EST‐derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. • Conclusions: The novel markers described here will be useful for future genetic studies of P. sativum ; their amplification in lentil ( Lens culinaris ) demonstrates their potential for use in closely related species.